Using isoelectric point to determine the pH for initial protein crystallization trials
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چکیده
منابع مشابه
Using isoelectric point to determine the pH for initial protein crystallization trials
MOTIVATION The identification of suitable conditions for crystallization is a rate-limiting step in protein structure determination. The pH of an experiment is an important parameter and has the potential to be used in data-mining studies to help reduce the number of crystallization trials required. However, the pH is usually recorded as that of the buffer solution, which can be highly inaccura...
متن کاملComment on 'Protein isoelectric point as a predictor for increased crystallization screening efficiency'
MOTIVATION Increased efficiency in initial crystallization screening reduces cost and material requirements in structural genomics. Because pH is one of the few consistently reported parameters in the Protein Data Bank (PDB), the isoelectric point (pI) of a protein has been explored as a useful indirect predictor for the optimal choice of range and distribution of the pH sampling in crystalliza...
متن کاملPIP-DB: the Protein Isoelectric Point database
UNLABELLED A protein's isoelectric point or pI corresponds to the solution pH at which its net surface charge is zero. Since the early days of solution biochemistry, the pI has been recorded and reported, and thus literature reports of pI abound. The Protein Isoelectric Point database (PIP-DB) has collected and collated these data to provide an increasingly comprehensive database for comparison...
متن کاملOptimum conditions for protein extraction from tuna processing by-products using isoelectric solubilization and precipitation processes
The by-product from tuna processing is a potential source of edible protein. Therefore, it is very important to extract protein from such raw materials for human food. In this study the optimum pH for protein extraction from tuna by-products was optimized by using isoelectric solubilization and precipitation processes. The Response Surface Methodology (RSM) and the single factor model were used...
متن کاملOptimum conditions for protein extraction from tuna processing by-products using isoelectric solubilization and precipitation processes
The by-product from tuna processing is a potential source of edible protein. Therefore, it is very important to extract protein from such raw materials for human food. In this study the optimum pH for protein extraction from tuna by-products was optimized by using isoelectric solubilization and precipitation processes. The Response Surface Methodology (RSM) and the single factor model were used...
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ژورنال
عنوان ژورنال: Bioinformatics
سال: 2015
ISSN: 1460-2059,1367-4803
DOI: 10.1093/bioinformatics/btv011